Annotated protein:	Protocadherin 7 (Protocadherin 7a). Gene symbol: PCDH7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A6IJE8
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SynGO gene info:	SynGO data @ PCDH7
Ontology domain:	Biological Process
SynGO term:	regulation of postsynapse assembly (GO:0150052)
Synapse type(s):	hippocampus, glutamatergic
Annotated paper:	Wang Y, et al. "PCDH7 interacts with GluN1 and regulates dendritic spine morphology and synaptic function" Sci Rep. 2020 Jul 2;10(1):10951 PMID:32616769
Figure(s):	Figure 4
Annotation description:	Figure 3: Knockdown of PCDH7 changes dendritic spine morphology in dissociated neurons. "Dissociated neurons were transfected with either scrambled shRNA, or two distinct shRNAs targeting PCDH7 (together with GFP as a marker protein), and morphology and density of dendritic spines were analyzed. Neurons with reduced PCDH7 either by shRNA_A or shRNA_C showed similar phenotype with longer dendritic spines (Fig. 3B,C). We found that although the total dendritic spine density was not affected by PCDH7 knockdown, there was around 50% reduction in the density of typical spines (length < 2 μm), and about a threefold increase in the density of longer spines (length > 2 μm) (Fig. 3D)." Figure 4: Knocking down PCDH7 modestly increases dendritic spine length in hippocampal slices with no significant impact on NMDA current.
Evidence tracking, Biological System:	Cultured neurons
Evidence tracking, Protein Targeting:	RNAi / shRNA
Evidence tracking, Experiment Assay:	Confocal
Annotator(s):	Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	5350
Dataset release (version):	20231201
View annotation as GO-CAM model: