| Annotated protein: | E3 ubiquitin-protein ligase RNF34 (EC 2.3.2.27) (RING finger protein 34) (RING finger protein MOMO) (RING-type E3 ubiquitin transferase RNF34). Gene symbol: RNF34. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6AYH3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RNF34 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Jin H, et al. "Ring finger protein 34 (RNF34) interacts with and promotes gamma-aminobutyric acid type-A receptor degradation via ubiquitination of the gamma2 subunit" J Biol Chem. 2014 Oct 17;289(42):29420-36 PMID:25193658 |
| Figure(s): | Figure 8, 9 |
| Annotation description: | Figure 8: Cultured hippocampal neurons overexpressing RNF34 show reduced density of endogenous γ2-GABAAR clusters and reduced GABAergic innervations. "These results show that overexpression of RNF34 significantly decreases the number of γ2-GABAAR clusters in the transfected neurons and the number of GABAergic contacts that the transfected neurons receive, and these effects depend on the E3 UBL activity of RNF34." Figure 9: Knocking down endogenous RNF34 in cultured hippocampal neurons increases both the γ2-GABAAR cluster density and GABAergic innervation. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5348 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |