| Annotated protein: | E3 ubiquitin-protein ligase RNF34 (EC 2.3.2.27) (RING finger protein 34) (RING finger protein MOMO) (RING-type E3 ubiquitin transferase RNF34). Gene symbol: RNF34. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6AYH3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RNF34 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic specialization (GO:0099572) |
| Synapse type(s): | cerebellum, GABAergic hippocampus, GABAergic |
| Annotated paper: | Jin H, et al. "Ring finger protein 34 (RNF34) interacts with and promotes gamma-aminobutyric acid type-A receptor degradation via ubiquitination of the gamma2 subunit" J Biol Chem. 2014 Oct 17;289(42):29420-36 PMID:25193658 |
| Figure(s): | Figure 7 |
| Annotation description: | Figure 7: immunofluorescence imaging of 21DIV cultured hippocampal neurons showed RNF34 co-localization with inhibitory synapse marker proteins GABA-A-gamma2 and GAD. Figure 8: immunogold EM of rat brain cerebellum showed RNF34 localization to both pre- and post-synapses of GABAergic synapses. Postsynaptic localization was observed at or adjacent to the postsynaptic specialization. Presynaptic localization appeared both at the active zone and further from the active zone / plasma membrane. "The EM immunogold data are consistent with the notion that RNF34 is localized at synapses (pre- and post-synaptically), nonsynaptic membranes, and cytoplasm, probably at cytoplasmic organelles such as endosomes. Thus, the immunofluorescence and immunogold data show that although RNF34 is not exclusively localized at GABAergic synapses, the latter frequently show the presence of RNF34. GABAAR clusters frequently have RNF34 associated with them." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5347 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |