| Annotated protein: | Ubiquitin carboxyl-terminal hydrolase 6 (EC 3.4.19.12) (Deubiquitinating enzyme 6) (Proto-oncogene TRE-2) (Ubiquitin thioesterase 6) (Ubiquitin-specific-processing protease 6). Gene symbol: USP6. Taxonomy: Homo sapiens (Human). Uniprot ID: P35125 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ USP6 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Zeng F, et al. "The deubiquitinase USP6 affects memory and synaptic plasticity through modulating NMDA receptor stability" PLoS Biol. 2019 Dec 16;17(12):e3000525 PMID:31841517 |
| Figure(s): | Figure 5, 6, 7 |
| Annotation description: | Figure 5: overexpression of deubiquitinating enzyme USP6 (construct described in Fig. 1c) in mouse cortex and hippocampus increased abundance levels for NMDAR subunits (GRIN1/2A/2B) and PSD95 (DLG4) but not AMPARs (GRIA1/2) nor synaptophysin. Figure 5d: overexpressed USP6 is present in synaptosomal and PSD biochemical fractions prepared from mouse cortex. Figure 6: USP6 interacts with NMDA receptors as demonstrated by coimmunoprecipitations in mouse brain lysates. Figure 7: USP6 attenuates NMDA receptor ubiquitination and enhances NMDA receptor stabilization. Figure 8: USP6 depletion reduces GluN1 expression in ESC-derived human excitatory neurons. Finally, authors summarise with a cartoon in Figure 9. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5340 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |