| Annotated protein: | Protein turtle homolog A (Dendrite arborization and synapse maturation protein 1) (Immunoglobulin superfamily member 9A) (IgSF9A). Gene symbol: IGSF9. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P0C5H6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IGSF9 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse maturation (GO:0090128) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Shi SH, et al. "The immunoglobulin family member dendrite arborization and synapse maturation 1 (Dasm1) controls excitatory synapse maturation" Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13346-51 PMID:15340156 |
| Figure(s): | Figure 5 |
| Annotation description: | Dasm1 Controls Synapse Maturation "One emerging model of excitatory synapse maturation is the "silent synapse" hypothesis (2-5). In neurons in which Dasm1 function is perturbed by expressing Dasm1 RNAi, the cytoplasmic tail deletion mutant, or the isolated cytoplasmic tail, we found that AMPA-R-mediated, but not NMDA-R-mediated, synaptic transmission is specifically reduced, raising the possibility that Dasm1 is involved in synapse maturation, specifically the activation of silent synapses. This possibility was pursued by examining the fraction of synaptic transmission failures (failure rate) evoked with minimal stimulation at both hyperpolarized (-60 mV) and depolarized (+55 mV) potentials in neurons expressing the dominant negative proteins [either Dasm1(delC)EGFP or EGFP-Dasm1(C200)] and nearby uninfected control neurons (Fig. 5). Compared with nearby uninfected control neurons, the evoked synaptic transmission onto neurons expressing Dasm1(delC)-EGFP (Fig. 5A) showed a greater failure rate when neurons were clamped at the -60 mV, but not when clamped at +55 mV. Thus, there are more silent synapses in neurons expressing Dasm1(delC)-EGFP (uninf, 21 ± 6%, n = 39; inf, 53 ± 7%, n = 37; P < 0.001) (Fig. 5C). Similar results were obtained for EGFP-Dasm1(C200) (uninf, 28 ± 4%, n = 20; inf, 48 ± 5%, n = 27; P < 0.01) (Fig. 5 B and D). These results suggest that Dasm1 is involved in "awakening" the silent synapses and thus promoting synaptic maturation." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5338 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |