Annotated protein: | Protein turtle homolog A (Dendrite arborization and synapse maturation protein 1) (Immunoglobulin superfamily member 9A) (IgSF9A). Gene symbol: IGSF9. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P0C5H6 |
antibody wiki: | |
SynGO gene info: | SynGO data @ IGSF9 |
Ontology domain: | Cellular Component |
SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
Synapse type(s): | hippocampus, glutamatergic |
Annotated paper: | Shi SH, et al. "The immunoglobulin family member dendrite arborization and synapse maturation 1 (Dasm1) controls excitatory synapse maturation" Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13346-51 PMID:15340156 |
Figure(s): | Figure 1 |
Annotation description: | "Double labeling of hippocampal neurons cultured for ~4 weeks with antibodies against Dasm1 and glutamate receptor 2/4 (GluR2/4) revealed a partial overlap between Dasm1 and GluR2/4 in a punctate pattern along the dendrites, especially at spine-like structures (Fig. 1A, arrowheads), suggesting that Dasm1 is localized at the excitatory synapses in mature neurons. Furthermore, subcellular fractionation of adult rat brains showed that Dasm1 was abundant in the PSD, in which postsynaptic proteins such as PSD-95 and NR2B were enriched (Fig. 1B)." Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
Evidence tracking, Biological System: | Cultured neurons |
Evidence tracking, Protein Targeting: | Antibody (detection) |
Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
SynGO annotation ID: | 5337 |
Dataset release (version): | 20231201 |
View annotation as GO-CAM model: |