| Annotated protein: | OL-protocadherin isoform (Protocadherin 10). Gene symbol: PCDH10. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q925I8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PCDH10 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Tsai NP, et al. "Multiple autism-linked genes mediate synapse elimination via proteasomal degradation of a synaptic scaffold PSD-95" Cell. 2012 Dec 21;151(7):1581-94 PMID:23260144 |
| Figure(s): | Figure 1H |
| Annotation description: | "We also determined whether Pcdh10 is present in dendritic spines and localized to excitatory synapses by performing immunohistochemistry for Pcdh10 and the postsynaptic excitatory synapse marker, PSD-95 in dissociated hippocampal neurons that were transfected with GFP to visualize dendritic spines. We observed that Pcdh10 was present in dendritic spines where PSD-95 was also enriched (Figure 1H). To validate the specificity of the antibody used for immunohistochemistry, we performed staining on hippocampal neurons transfected with two independent shRNAs against Pcdh10 (Figure S1C). The results showed similar knock-down efficiency (>50%) by shRNAs as observed with Western blot (Figures 3F and S3B), validating the fluorescence signal of Pcdh10 seen in Fig. 1H. Therefore, Pcdh10 is present in spines at the site of excitatory synapses and may function in MEF2 regulation of excitatory synapse number." Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5329 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |