| Annotated protein: | Neurogenic locus notch homolog protein 2 (Notch 2) [Cleaved into: Notch 2 extracellular truncation; Notch 2 intracellular domain]. Gene symbol: NOTCH2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9QW30 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NOTCH2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Loh KH, et al. "Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts" Cell. 2016 Aug 25;166(5):1295-1307.e21 PMID:27565350 |
| Figure(s): | Figure 4, 5 |
| Annotation description: | Figure 4: proximity labeling of synaptic cleft proteins identified NOTCH2. Figure 5e: NOTCH2 was also identified in western blots of synaptosome fractions prepared from adult rat brain. Figure 5g,h: immunofluorescence labeling of endogenous NOTCH2 showed partial localization apposed to Bassoon in both cultured cortical neurons and adult rat brain tissue. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal Western blot Mass-spectrometry |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5328 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |