| Annotated protein: | Semaphorin-4C (M-Sema F) (Semaphorin-C-like 1) (Sema I) (Semaphorin I). Gene symbol: SEMA4C. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q64151 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SEMA4C |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Inagaki S, et al. "Sema4c, a transmembrane semaphorin, interacts with a post-synaptic density protein, PSD-95" J Biol Chem. 2001 Mar 23;276(12):9174-81 PMID:11134026 |
| Figure(s): | Figure 6, 7 |
| Annotation description: | Figure 4: In vitro analysis showing the interaction of PDZ domains of PSD95 (DLG4) with Sema4C. In cerebral cortex, these proteins also coimmunoprecipitate (Fig. 7a). Figure 6: Sema4C and PSD95 (DLG4) are enriched in PSD biochemical fractions prepared from cerebral cortex tissue. Figure 7c: confocal imaging of immunofluorescence labeling for Sema4C and PSD-95 (DLG4) in cultured cortical neurons showed co-localization in synaptic puncta. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5318 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |