| Annotated protein: | Semaphorin-4D (M-Sema G) (Semaphorin-C-like 2) (Semaphorin-J) (Sema J) (CD antigen CD100). Gene symbol: SEMA4D. Taxonomy: Mus musculus (Mouse). Uniprot ID: O09126 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SEMA4D |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse assembly (GO:0051963) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Raissi AJ, et al. "Sema4D localizes to synapses and regulates GABAergic synapse development as a membrane-bound molecule in the mammalian hippocampus" Mol Cell Neurosci. 2013 Nov;57:23-32 PMID:24036351 |
| Figure(s): | Figure 2, 4, 6 |
| Annotation description: | Figure 5: in biochemical fractions prepared from rat hippocampal tissue, Sema4D is enriched in P2 as compared to homogenate and in synaptic membranes as compared to P2 (homogenate < P2 < LP1). Figure 2: The extracellular domain of Sema4D is required for GABAergic synapse formation ( but not the intracellular C-terminus, Fig. 1). Figure 4: Sema4D promotes GABAergic synaptogenesis as a membrane bound molecule. Figure 6: Sema4D is required for proper GABAergic but not glutamatergic synapse development. authors conclude: "Because Sema4D does not require its intracellular domain to regulate synapse formation, it is possible that Sema4D does not need to interact with intracellular scaffolding proteins nor undergo intracellular post-translational modifications such as phosphorylation to regulate GABAergic synapse development. We postulate that the specificity of Sema4D activity arises via interaction(s) with receptor(s) on the appropriate presynaptic interneuron. Indeed, the high affinity Sema4D receptor PlexinB1 (Tamagnone et al. 1999) is required for the ability of the soluble, extracellular domain of Sema4D to promote GABAergic synapse formation (Kuzirian et al. 2013). Thus, we favor a model whereby Sema4D, localized to the postsynaptic membrane of GABAergic synapses, promotes synapse formation via a trans-synaptic interaction with PlexinB1 expressed on the presynaptic terminal of GABAergic interneurons." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5317 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |