| Annotated protein: | E3 ubiquitin-protein ligase RNF138 (EC 2.3.2.27) (RING finger protein 138) (RING-type E3 ubiquitin transferase RNF138). Gene symbol: RNF138. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q99PD2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RNF138 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | forebrain cerebral cortex, glutamatergic |
| Annotated paper: | Fu SJ, et al. "Ubiquitin Ligase RNF138 Promotes Episodic Ataxia Type 2-Associated Aberrant Degradation of Human Ca(v)2.1 (P/Q-Type) Calcium Channels" J Neurosci. 2017 Mar 1;37(9):2485-2503 PMID:28167673 |
| Figure(s): | Figure 2 |
| Annotation description: | "Figure 2A shows that, in rat forebrain lysates, endogenous RNF138 was coimmunoprecipitated with CaV2.1, consistent with the idea that the two molecules coexist in the same protein complex in native neurons." "As depicted in Figure 2B, both RNF138 and CaV2.1 are highly enriched in the SPM fraction, as well as in the SPM subfractions PSD I and PSD II, implying a colocalization of the two proteins at presynaptic and postsynaptic compartments." "Compared with CaV2.1, RNF138 displays a diffuse immunofluorescence pattern in both axonal and dendrosomatic regions (Fig. 2E, right). Nevertheless, like CaV2.1, RNF138 also shows prominent patterns of punctate staining at presynaptic and postsynaptic sites. Furthermore, CaV2.1 and RNF138 display quantitatively comparable colocalization with the synaptic proteins synaptophysin and PSD-95 (Fig. 2F)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5311 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |