| Annotated protein: | Voltage-dependent P/Q-type calcium channel subunit alpha-1A (Brain calcium channel I) (BI) (Calcium channel, L type, alpha-1 polypeptide isoform 4) (Voltage-gated calcium channel subunit alpha Cav2.1). Gene symbol: CACNA1A. Taxonomy: Mus musculus (Mouse). Uniprot ID: P97445 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA1A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | cerebellum |
| Annotated paper: | Kulik A, et al. "Immunocytochemical localization of the alpha 1A subunit of the P/Q-type calcium channel in the rat cerebellum" Eur J Neurosci. 2004 Apr;19(8):2169-78 PMID:15090043 |
| Figure(s): | Figure 2, 4, 5 |
| Annotation description: | Extensive immunoelectron microscopy Cav2.1 (CACNA1A) was performed. Knockout mice were used to validate specificity. "Postsynaptically, virtually all PC spines were immunoreactive for the α1A protein. Peroxidase reaction end-product for the channel subunit accumulated in dendritic spines postsynaptic to PF (Fig. 2C) and CF (Fig. 2D) varicosities." "At the electron microscopic level, the most intense immunoreactivity for the α1A subunit was found in the presynaptic active zone of parallel fibre varicosities. The dendritic spines of Purkinje cells were also strongly labelled with the highest density of immunoparticles detected within 180 nm from the edge of the asymmetrical parallel fibre-Purkinje cell synapses." Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5310 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |