Annotated protein: | Fibroblast growth factor 22 (FGF-22). Gene symbol: FGF22. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9ESS2 |
antibody wiki: | |
SynGO gene info: | SynGO data @ FGF22 |
Ontology domain: | Biological Process |
SynGO term: | regulation of synapse maturation (GO:0090128) |
Synapse type(s): | hippocampus, GABAergic |
Annotated paper: | Terauchi A, et al. "Distinct FGFs promote differentiation of excitatory and inhibitory synapses" Nature. 2010 Jun 10;465(7299):783-7 PMID:20505669 |
Figure(s): | Figure 2, 3 |
Annotation description: | Figure 2: FGF22 knockout mice have impaired synapse maturation/differentiation. Figure 3a-d: "Overexpression of FGF22 and FGF7 in cultured hippocampal neurons leads to an increase in the clustering of VGLUT1 and VGAT puncta, respectively, on the FGF-expressing neuron (Fig. 3a). This suggests that FGF22 and FGF7 specifically promote differentiation of glutamatergic and GABAergic presynaptic terminals, respectively, as target-derived molecules." Figure 3e-j: immunofluorescence imaging of FGF22 in cultured hippocampal neurons showed co-localization with excitatory postsynapse marker protein PSD95 (DLG4). Same results for both overexpressed FGF22-EGFP and endogenous FGF22. FGF22 did not colocalize with inhibitory synapse marker proteins. |
Evidence tracking, Biological System: | Cultured neurons |
Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression Antibody (detection) |
Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
SynGO annotation ID: | 5308 |
Dataset release (version): | 20231201 |
View annotation as GO-CAM model: |