| Annotated protein: | Cell adhesion molecule 2 (Immunoglobulin superfamily member 4D) (IgSF4D) (Nectin-like protein 3) (NECL-3) (Synaptic cell adhesion molecule 2) (SynCAM 2). Gene symbol: CADM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q1WIM2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CADM2 |
| Ontology domain: | Biological Process |
| SynGO term: | synapse adhesion between pre- and post-synapse (GO:0099560) |
| Synapse type(s): | hippocampus, glutamatergic hippocampus, GABAergic forebrain |
| Annotated paper: | Fogel AI, et al. "SynCAMs organize synapses through heterophilic adhesion" J Neurosci. 2007 Nov 14;27(46):12516-30 PMID:18003830 |
| Figure(s): | Figures 4-9 |
| Annotation description: | Figure 4a: SynCAM 2 (CADM2) in enriched in synaptic plasma membrane biochemical fractions prepared from rat forebrain at P9 (as compared to e.g. synaptosomes). Figure 4b: confocal imaging of immunofluorescence labeling for SynCAM 2 (CADM2), GAD65 (GAD2) and PSD-95 (DLG4) in cultured hippocampal neurons from showed SynCAM 2 is localized to both inhibory and excitatory synapses. Figure 5, 6: Homophilic cell adhesive interactions of SynCAM 2, and heterophilic interactions with SynCAM 1, are shown by cell-cell contact analysis in HEK293 cells. Figure 7: Heterophilic SynCAM binding is mediated by the first two Ig-like domains and is controlled by N-glycosylation. Figure 8: SynCAM 1 and 2 coimmunoprecipitate in synaptosomes prepared from rat forebrain at P15. Figure 9: in mixed coculture assay of HEK 293 cells expressing SynCAM 1 or 2 seeded atop dissociated hippocampal cultures, SynCAM 1 and 2 recruit presynaptic marker proteins and engage each other in adhesive neuronal complexes. |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5300 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |