| Annotated protein: | Ras-related C3 botulinum toxin substrate 3 (EC 3.6.5.2) (p21-Rac3). Gene symbol: RAC3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P60764 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAC3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Pennucci R, et al. "Rac1 and Rac3 GTPases differently influence the morphological maturation of dendritic spines in hippocampal neurons" PLoS One. 2019 Aug 1;14(8):e0220496 PMID:31369617 |
| Figure(s): | Figure 4 |
| Annotation description: | Figure 3: overexpression of GFP-Rac3 showed localization to dendritic shaft and spines (co-localizatoin with endogenous Homer) in 4DIV hippocampal neurons. Figure 4: double knockout of Rac1 and Rac3 leads to severely impaired postsynapse formation in 4DIV hippocampal neurons, which could be rescued by overexpression of either GFP-Rac3 or GFP-Rac1. authors summarise; "The re-expression of either GTPase significantly and strongly increased the size of mushroom spines, as detected by measuring the area of their heads (Fig 4D): Rac1 and Rac3 expression induced a 3.3-fold (P < 0.001) and 5.4-fold (P < 0.001) increase in average spine head area respectively, compared to the average spine head area for the few spines formed by the double KO neurons. Interestingly, as already observed after overexpressing either GTPase in the WT neurons, Rac3 has a stronger effect compared to Rac1 on the size of the spines. In the case of re-expression of the Rac3 GTPase in double KO cells the effects were more evident compared to its overexpression in WT cells that express endogenous Rac3. The increase of the mean spine head area induced by Rac3 was 1.6-fold that induced by the re-expression of Rac1 (P < 0.001). Altogether these data indicate that the re-expression of either Rac1 or Rac3 was sufficient to re-establish the formation of dendritic spines, and that Rac3 promoted the formation of larger spines." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5297 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |