| Annotated protein: | SLIT-ROBO Rho GTPase-activating protein 3 (srGAP3) (Rho GTPase-activating protein 14) (WAVE-associated Rac GTPase-activating protein) (WRP). Gene symbol: SRGAP3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q812A2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SRGAP3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Carlson BR, et al. "WRP/srGAP3 facilitates the initiation of spine development by an inverse F-BAR domain, and its loss impairs long-term memory" J Neurosci. 2011 Feb 16;31(7):2447-60 PMID:21325512 |
| Figure(s): | Figure 5, 6 |
| Annotation description: | Figure 5: "Endogenous WRP (SRGAP3) protein is expressed throughout the developmental time period of synaptogenesis (supplemental Fig. S3G). Thus the role of WRP during spinogenesis was tested in vitro (Fig. 5) by transfecting cultured neurons from WRPflox/flox mice with GFP-Cre and the soluble fill tdTomato at different time points and then performing image analysis to assess the effect on spine formation and development. ... Transfection of Cre at DIV5 (before dendritic filopodial formation) resulted in a 20% reduction in spine density (Fig. 5E-G). In contrast, transfection of Cre at DIV9 (after dendritic filopodia appear) had no significant effect on spine density (Fig. 5H-J). Together, these results strongly support the hypothesis that WRP is required during the early (filopodial) stages of spine development." Figure 6: WRP (SRGAP3) regulates mushroom spine development but not maintenance in vivo. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5291 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |