| Annotated protein: | Capping protein, Arp2/3 and myosin-I linker protein 3 (Capping protein regulator and myosin 1 linker protein 3) (Leucine-rich repeat-containing protein 16B). Gene symbol: CARMIL3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3UFQ8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CARMIL3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Spence EF, et al. "In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation" Nat Commun. 2019 Jan 23;10(1):386 PMID:30674877 |
| Figure(s): | Figure 5, 6 |
| Annotation description: | Figure 5: CARMIL3 depletion causes alterations in dendritic spine maturation. " However, at DIV16 (Fig. 5e), a time point when the majority of developing excitatory postsynaptic structures are spines, there was a significant shift in the morphology of CARMIL3-depleted (MUT) neurons compared with control (WT) neurons. The density of dendritic spines was decreased and there were more filopodia-like protrusions in CARMIL3-depleted neurons (Fig. 5f, g)." Figure 6: Depletion of CARMIL3 alters excitatory synaptic transmission "There was a 51% reduction in the GluA1-GluA2 surface enrichment within dendritic protrusions from gene-trapped neurons compared with WT neurons (Fig. 6g). This difference was driven by a reduction in surface enrichment specifically in dendritic spines, as filopodia in both groups had similarly low levels of enrichment (Fig. 6h)." authors summarise; "Using new CRISPR-based approaches, we validate that endogenous CARMIL3 is localized to developing synapses where it facilitates the recruitment of capping protein and is required for spine structural maturation and AMPAR recruitment associated with synapse unsilencing." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5290 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |