| Annotated protein: | Caskin-1 (CASK-interacting protein 1). Gene symbol: CASKIN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6P9K8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CASKIN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Bencsik N, et al. "Dendritic spine morphology and memory formation depend on postsynaptic Caskin proteins" Sci Rep. 2019 Nov 14;9(1):16843 PMID:31727973 |
| Figure(s): | Figure 5, 7 |
| Annotation description: | Figure 5: "Overexpressed Caskin1 was detected predominantly within the somatodendritic region of the transfected neurons, with local enrichment within the dendritic spine heads (Fig. 5F,G). Ultrastructural analyses in Caskin1-V5 and EGFP cotransfected neurons were processed by anti-V5 pre-embedding immunocytochemistry and visualized by DAB precipitates. Our results proved that overexpressed Caskin1 was indeed present in postsynaptic dendritic spines forming synapses (see Fig. 5E)." Figure 7: in immunofluorescence imaging of Caskin-1 and -2 double-knockout mice, overexpressed EGFP-Caskin1 co-localized with endogenous Shank2 at dendritic spines. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Figure 6: STED super-resolution microscopy showed partial co-localization between CASKIN1 and PSD95 (DLG4) @ PMID:36217825 |
| SynGO annotation ID: | 5283 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |