| Annotated protein: | P2X purinoceptor 2 (P2X2) (ATP receptor) (Purinergic receptor). Gene symbol: P2RX2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P49653 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ P2RX2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Masin M, et al. "Fe65 interacts with P2X2 subunits at excitatory synapses and modulates receptor function" J Biol Chem. 2006 Feb 17;281(7):4100-8 PMID:16330549 |
| Figure(s): | Figure 5 |
| Annotation description: | Figure 5: "The adaptor protein Fe65 (APBB1) is present in Schaffer collaterals/CA1 dendritic spine hippocampal synapses where it colocalizes with P2X2 subunits. ... H-K, electron micrographs showing double immunogold labeling for P2X2 subunits (5 nm) and Fe65 (10 nm). Only the postsynaptic specialization of CA1 synapses presented labeling for both proteins" Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | P2X2 antibody reference and EM localization to the postsynapse @ PMID:11160443 |
| SynGO annotation ID: | 5279 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |