| Annotated protein: | Alpha-actinin-4 (Non-muscle alpha-actinin 4). Gene symbol: ACTN4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9QXQ0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ACTN4 |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of postsynaptic actin cytoskeleton (GO:0098973) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Kalinowska M, et al. "Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors" J Biol Chem. 2015 Jun 26;290(26):15909-20 PMID:25944910 |
| Figure(s): | Figure 8 |
| Annotation description: | Figure 8: α-Actinin-4 (ACTN4) regulates spine morphogenesis in cultured cortical neurons. Overexpressed Actn4 localized to dendritic spine heads and increased spine density and head size. A truncation mutant of Actn4 that lacked the crucial C-terminal domain increased spine/protrusion length and spine density but not spine width. "Collectively, these findings provide evidence for a role of Actn4 in the regulation of spine morphogenesis and suggest that this function involves interaction with CaMKII." authors discuss: "Our studies identify Actn4 as a novel group 1 mGluR interacting partner and a critical cell-autonomous effector of protrusion dynamics that is necessary for group 1 mGluR-dependent spine remodeling. We find that group 1 mGluR activation enhances protrusion dynamics by inducing elongation and turnover. These effects are abrogated by Actn4 silencing in the absence of perturbations in receptor expression at the neuronal surface or signaling capacity. In addition, we discover a role for Actn4 as a central player in processes underlying protrusion dynamics. ... Native actinins form homodimers that bind F-actin within the cortical actin meshwork and can be transiently tethered to plasma membrane microdomains via interaction with phosphoinositides (29). Therefore, Actn4 may facilitate the functional interaction of membrane mGluRs with cortical F-actin and signaling proteins that contribute to the modification of the actin cytoskeleton." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5276 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |