Annotated protein:E3 ubiquitin-protein ligase CBL-B (EC 2.3.2.27) (Casitas B-lineage lymphoma proto-oncogene b) (RING-type E3 ubiquitin transferase CBL-B) (SH3-binding protein CBL-B) (Signal transduction protein CBL-B). Gene symbol: CBLB. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3TTA7
antibody wiki:
SynGO gene info:SynGO data @ CBLB
Ontology domain:Biological Process
SynGO term:regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149)
Synapse type(s):spinal cord, glutamatergic
Annotated paper:Zhang ZY, et al. "Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn" Sci Signal. 2020 Jun 30;13(638):eaaw1519 PMID:32606037
Figure(s):Figures 1-4
Annotation description:Figure 1b: confocal imaging of immunofluorescence labeling for Cbl-b and PSD-95 (DLG4) in cultured spinal cord dorsal horn neurons showed partial co-localization. Imaging resolution was too low to pinpoint precise synaptic localization.

Figure 2: Cbl-b specifically interacts with NMDAR subunit GluN2B (GRIN2B) in co-immunoprecipitations from spinal cord dorsal horn of adult mice and HEK293T cells.

Figure 3: Cbl-b enhanced the ubiquitination and reduced the surface expression of GluN2B in spinal neuron cultures and HEK293T cells.

Figure 4: Cbl-b promotes the developmental decline of synaptic GluN2B receptors.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:5273
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology