Annotated protein:	E3 ubiquitin-protein ligase CBL-B (EC 2.3.2.27) (Casitas B-lineage lymphoma proto-oncogene b) (RING-type E3 ubiquitin transferase CBL-B) (SH3-binding protein CBL-B) (Signal transduction protein CBL-B). Gene symbol: CBLB. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3TTA7
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SynGO gene info:	SynGO data @ CBLB
Ontology domain:	Biological Process
SynGO term:	regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149)
Synapse type(s):	spinal cord, glutamatergic
Annotated paper:	Zhang ZY, et al. "Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn" Sci Signal. 2020 Jun 30;13(638):eaaw1519 PMID:32606037
Figure(s):	Figures 1-4
Annotation description:	Figure 1b: confocal imaging of immunofluorescence labeling for Cbl-b and PSD-95 (DLG4) in cultured spinal cord dorsal horn neurons showed partial co-localization. Imaging resolution was too low to pinpoint precise synaptic localization. Figure 2: Cbl-b specifically interacts with NMDAR subunit GluN2B (GRIN2B) in co-immunoprecipitations from spinal cord dorsal horn of adult mice and HEK293T cells. Figure 3: Cbl-b enhanced the ubiquitination and reduced the surface expression of GluN2B in spinal neuron cultures and HEK293T cells. Figure 4: Cbl-b promotes the developmental decline of synaptic GluN2B receptors.
Evidence tracking, Biological System:	Cultured neurons
Evidence tracking, Protein Targeting:	Antibody (detection)
Evidence tracking, Experiment Assay:	Confocal
Annotator(s):	Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	5273
Dataset release (version):	20231201
View annotation as GO-CAM model: