Annotated protein:E3 ubiquitin-protein ligase MIB2 (EC 2.3.2.27) (Mind bomb homolog 2) (RBSC-skeletrophin/dystrophin-like polypeptide) (RING-type E3 ubiquitin transferase MIB2). Gene symbol: MIB2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q68LP1
antibody wiki:
SynGO gene info:SynGO data @ MIB2
Ontology domain:Cellular Component
SynGO term:postsynapse (GO:0098794)
Synapse type(s):hippocampus, glutamatergic
Annotated paper:Jurd R, et al. "Mind bomb-2 is an E3 ligase that ubiquitinates the N-methyl-D-aspartate receptor NR2B subunit in a phosphorylation-dependent manner" J Biol Chem. 2008 Jan 4;283(1):301-310 PMID:17962190
Figure(s):Figure 6
Annotation description:Figure 6: confocal imaging of immunofluorescence labeling for Mind Bomb-2 (MIB2) and PSD-95 (DLG4) in cultured rat hippocampal neurons showed partial co-localization.

Figure 7: Mib2 associates with the NR2B subunit in the hippocampus.
"forskolin treatment resulted in an increase in NR2B phosphorylation (lanes 1 versus 2, top panel), and a corresponding increase in Mib2 co-immunoprecipitation with the NR2B subunit (lanes 1 versus 2, bottom panel). Similar results were obtained when KCl was added at the last 5 min of forskolin treatment to mimic brief neuronal stimulation (Fig. 7, lanes 3 versus 1 and 2). Together these results suggest that in the hippocampus, Mib2 binds NR2B in an activity-dependent manner."
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Confocal
IP + WB/MSMS
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:5268
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology