| Annotated protein: | Sushi domain-containing protein 4. Gene symbol: SUSD4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BH32 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SUSD4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Gonzalez-Calvo I, et al. "Sushi domain-containing protein 4 controls synaptic plasticity and motor learning" Elife. 2021 Mar 4;10:e65712 PMID:33661101 |
| Figure(s): | Figure 1, Figure 4 suppl. 2 |
| Annotation description: | Figure1: "To identify the subcellular localization of the SUSD4 protein and because of the lack of suitable antibodies for immunolabeling, viral particles enabling CRE-dependent coexpression of HA-tagged SUSD4 and GFP in neurons were injected in the cerebellum of adult mice expressing the CRE recombinase specifically in cerebellar PCs. Immunofluorescent labeling against the HA tag demonstrated the localization of HA-SUSD4 in dendrites and in some of the numerous dendritic spines present on the surface of distal dendrites (Figure 1C). These spines are the postsynaptic compartments of PF synapses in PCs. Immunofluorescence analysis of transduced cultured PCs showed that HA-tagged SUSD4 could be immunolabeled in non-permeabilizing conditions and located at the surface of dendrites and spines (Figure 1D). Double labeling with the postsynaptic marker GluD2 (GRID2) further showed partial colocalization at the surface of some, but not all, spines." Figure 4, supplement 2: immunofluorescence labeling of GFP-SUSD4 and endogenous GluA2 (GRIA2) showed partial co-localization at postsynapses of 13DIV mouse hippocampal neurons. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5259 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |