| Annotated protein: | NEDD8-conjugating enzyme Ubc12 (EC 2.3.2.34) (NEDD8 carrier protein) (Ubiquitin-conjugating enzyme E2 M). Gene symbol: UBE2M. Taxonomy: Mus musculus (Mouse). Uniprot ID: P61082 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ UBE2M |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Vogl AM, et al. "Neddylation inhibition impairs spine development, destabilizes synapses and deteriorates cognition" Nat Neurosci. 2015 Feb;18(2):239-51 PMID:25581363 |
| Figure(s): | Figure 4 |
| Annotation description: | Figure 4: NEDD8-conjugating enzyme Ubc12 (UBE2M) is present in pre- and post-synaptic biochemical fractions from mouse hippocampi. Blotting all neddylated proteins showed many Ubc12 targets in pre- and post-synapse. Furthermore, authors show that neddylation (which directly involves Ubc12) takes place at the synapse. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5253 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |