| Annotated protein: | Neuropilin-1 (A5 protein) (CD antigen CD304). Gene symbol: NRP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P97333 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NRP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Morita A, et al. "Regulation of dendritic branching and spine maturation by semaphorin3A-Fyn signaling" J Neurosci. 2006 Mar 15;26(11):2971-80 PMID:16540575 |
| Figure(s): | Fig. 4. |
| Annotation description: | Fig. 4, NRP1 colocalize with clusters of PSD-95 and on spine-like protrusions on dendrites. NRP1 is the receptor for Sema3A. Literal: "we performed double staining with anti-PSD95 and NRP-1 antibodies in the cultured neurons. We found that some of the NRP-1-positive structures were colocalized with PSD-95 clusters and the other in close contact with spine-like protrusions (Fig. 4). When the cultured cortical neurons were exposed to Sema3A, the cluster size of PSD-95 was markedly enhanced (Fig. 4b,d). ... An extensive colocalization of PSD-95 and NRP-1 or actin-rich protrusion was seen. The percentages of double-positive spots with and without Sema3A were 66.9 +/-9.3 and 53.9 +/- 5.6%, respectively (Fig. 4d)." 6/11/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 525 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |