| Annotated protein: | Acid-sensing ion channel 1 (ASIC1) (Amiloride-sensitive cation channel 2, neuronal) (Brain sodium channel 2) (BNaC2). Gene symbol: ASIC1. Taxonomy: Homo sapiens (Human). Uniprot ID: P78348-2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ASIC1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Song XL, et al. "Postsynaptic Targeting and Mobility of Membrane Surface-Localized hASIC1a" Neurosci Bull. 2021 Feb;37(2):145-165 PMID:32996060 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 2: confocal imaging of overexpressed human HA-tagged ASIC1a showed surface-expressed-ASIC1 at the postsynaptic density of cultured cortical neurons in close proximity to PSD95 (DLG4) and GluN1 (GRIN1). "Under non-permeabilized conditions, the HA labeling was clustered on the PM of somata, dendritic shafts, and spines (Fig. 2A), but not axons (Fig. 2A), consistent with the distribution of total hASIC1a (Fig. S1C and D). ... Interestingly, by co-immunostaining with the excitatory post-synaptic markers PSD-95 and the NMDA receptor subunit GluN1 (Fig. 2B and C), we found that the surface hASIC1a clusters in spine heads were closely adjacent to or partially co-localized with PSD-95 or GluN1 puncta (Fig. 2D and E). ... These observations indicate that hASIC1a is present at the surface of excitatory synapses, closely associated with the post-synaptic density region, in line with its role in excitatory synaptic function." Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5248 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |