| Annotated protein: | Striatin. Gene symbol: STRN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P70483 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STRN |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic structure (GO:0099159) |
| Synapse type(s): | striatum |
| Annotated paper: | Li D, et al. "Striatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons" J Biol Chem. 2018 Jul 13;293(28):11179-11194 PMID:29802198 |
| Figure(s): | Figure 6 |
| Annotation description: | Figure 6: Knockdown of striatin-1 causes an increase in density of stubby spines. "We examined the density of each type of spine in 40-μm segments of dendrites by immunostaining with GFP, comparing neurons transfected with the striatin-1 shRNA (Fig. 6a) with neurons transfected with the scrambled control (Fig. 6b). Knockdown of striatin-1 resulted in an increase in spine number per 40 μm (Fig. 6, c, left two plots, and d) that was due primarily to an increase in stubby spines (Fig. 6f). No significant difference was observed in the density of thin or mushroom spines (Fig. 6, g and h). Co-expression of the shRNA-insensitive striatin-1 cDNA prevented the effects of the shRNA, confirming the specificity of this effect (Fig. 6, c, right two plots, e, and f)." Author summary; "Reduced expression of striatin-1 resulted in increased dendritic complexity that was prevented by co-expression of an shRNA-insensitive striatin-1 cDNA. Expression of mutant PP2A-binding-deficient or Mob3-binding-deficient striatin-1 constructs could not rescue this phenotype of dendritic complexity. We also observed an increase in density of dendritic spines following reduction of striatin-1, particularly in immature stubby spines, that was also prevented by expression of the rescue construct. These observations suggest that striatin-1 contributes to maturation of striatal neurons by regulating dendritic morphology, likely through its role as a binding partner of PP2A. These effects were specific to striatal neurons, as knockdown of striatin-1 in cortical neurons did not result in significant changes in dendritic morphology. This is in line with the fact that striatin-1 is predominantly expressed in the striatum. However, despite these significant changes in dendritic arborization and spine density, functional connectivity and synaptic activity in striatal neurons did not appear to be affected by knockdown of striatin-1." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5241 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |