| Annotated protein: | Striatin 4. Gene symbol: STRN4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M6V8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STRN4 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic structure (GO:0099159) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Lin L, et al. "Determination of dendritic spine morphology by the striatin scaffold protein STRN4 through interaction with the phosphatase PP2A" J Biol Chem. 2017 Jun 9;292(23):9451-9464 PMID:28442576 |
| Figure(s): | Figure 5, 6, 7 |
| Annotation description: | Figure 5: shRNA knockdown of STRN4 in 15DIV hippocampal neurons resulted in the specific loss of mushroom spines, but not thin/stubby spines or filopodia. Figure 7: "To confirm the loss of mushroom spines that contain excitatory synapses, hippocampal neurons were co-transfected with the expression construct of PSD-95 intrabody to label the endogenous PSD-95 clusters (44), followed by SIM imaging. Knockdown of STRN4 significantly reduced the number of PSD-95-containing mushroom spines" Figure 6: "We found that STRN4 interacted with the catalytic subunit of PP2A, and this interaction was abolished by the STRN4 mutant that harbored the R116S/ R117E mutations (Fig. 6F). Next, RNAi-resistant wild-type or the PP2A-binding-deficient R116S/R117E mutant was introduced together with STRN4-shRNA into hippocampal neurons, and the effect on spine morphology of the transfected neurons was examined. Although co-expression of wild-type STRN4 reversed the loss of mushroom spines induced by the shRNA, the PP2A-binding-deficient STRN4 mutant failed to rescue the spine defects. Furthermore, compared with wildtype STRN4, co-expression of the PP2A-binding-deficient STRN4 mutant resulted in the formation of more filopodia (Fig. 7, A and B)." authors conclude; "Taken together, our findings collectively indicate that the tight regulation of PP2A by STRN4 in the STRIPAK complex is critical to govern dendritic spine morphology" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5240 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |