| Annotated protein: | Striatin 4. Gene symbol: STRN4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M6V8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ STRN4 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic hippocampus, GABAergic |
| Annotated paper: | Lin L, et al. "Determination of dendritic spine morphology by the striatin scaffold protein STRN4 through interaction with the phosphatase PP2A" J Biol Chem. 2017 Jun 9;292(23):9451-9464 PMID:28442576 |
| Figure(s): | Figure 1, 2, 3 |
| Annotation description: | Figure 1: confocal imaging of immunofluorescence labeling for STRN4 (aka. zinedin), PSD-95 (DLG4) and Gephyrin (GPHN) in cultured hippocampal neurons showed localization showed postsynaptic localization. "We found that STRN4 existed as discrete puncta in both the dendritic shaft and dendritic spines. The majority (78.1 ± 4.1%; 50 STRN4 puncta from three dendrites) of the spine-associated STRN4 puncta were co-localized with the excitatory postsynaptic protein PSD-95 (Fig. 1C). In contrast, relatively few (24.4 ± 5.6%; 46 puncta from two dendrites) of the non-spine-associated STRN4 puncta in the dendritic shaft were co-localized with the inhibitory postsynaptic scaffold protein gephyrin (Fig. 1D). These findings therefore indicate that STRN4 is mostly expressed at excitatory synapses." Figure 2,3: additional imaging data showed STRN4 co-localized at postsynaptic puncta. "We found that STRN4 expression differed in distinct subtypes of dendritic spines, with the highest STRN4 immunoreactivity present in stubby spines and the spine heads of mushroomshaped spines that contained PSD-95 clusters, whereas STRN4 immunoreactivity was significantly reduced in thin spines and filopodia (Fig. 3C)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | immunofluorescence staining showed STRN4/zinedin at postsynaptic puncta @ PMID:23015759 |
| SynGO annotation ID: | 5239 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |