| Annotated protein: | E3 ubiquitin-protein ligase Rnf220 (EC 2.3.2.27) (RING finger protein 220) (RING-type E3 ubiquitin transferase Rnf220). Gene symbol: RNF220. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6PDX6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RNF220 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ma P, et al. "RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission" Sci Adv. 2022 Sep 30;8(39):eabq4736 PMID:36179027 |
| Figure(s): | Figures 2-7 |
| Annotation description: | Figure 2: RNF220 is present in synaptosomal, but not PSD, biochemical fractions prepared from hippocampal tissue. RNF220-knockout significantly affected relative abundance levels of GluA1 and 2 (GRIA1, GRIA2). Figure 3: RNF220 regulates excitatory synaptic transmission and plasticity. "We next examined the regulation of synaptic plasticity by RNF220 by inducing LTP (long-term potentiation) of the CA1 pyramidal neurons from RNF220 cKO mice and control littermates. It was shown that the potentiation of AMPAR-mediated EPSCs was significantly impaired in RNF220 depletion neurons (RNF220 KO, 239.69 ± 22.94% baseline; control, 442.02 ± 51.04% baseline; Fig. 3D). Together, all these data indicate that RNF220 regulates postsynaptic AMPAR expression to affect excitatory synaptic transmission and plasticity." Figure 4: RNF220 directly ubiquitinates AMPARs to regulate excitatory synaptic activity. Figure 5: "Together, these results indicate that RNF220 is an E3 ubiquitin ligase for AMPARs to control protein stability and regulate excitatory synaptic transmission." Figure 6: RNF220 regulation of synaptic activity involves ubiquitination of both GluA1 and GluA2 receptors. Figure 7: "Together, these results indicate that the ubiquitination regulatory mechanism by RNF220 is also involved in neuronal surface expression of AMPARs." authors conclude: "In this study, we find that RNF220 is an E3 ubiquitin ligase for AMPARs to repress their expression on neuronal surface and synapse." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5234 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |