| Annotated protein: | Protein ERGIC-53 (ER-Golgi intermediate compartment 53 kDa protein) (Lectin mannose-binding 1) (p58). Gene symbol: LMAN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62902 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LMAN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic Golgi apparatus (GO:0150051) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Gurth CM, et al. "Synaptic activity and strength are reflected by changes in the post-synaptic secretory pathway" Sci Rep. 2020 Nov 25;10(1):20576 PMID:33239744 |
| Figure(s): | Figure S1 |
| Annotation description: | Figure S1d: STED super-resolution microscopy of hippocampal primary neurons with antibody labeling of endogenous ERGIC53 (an ER-Golgi intermediate compartment marker), postsynaptic density marker Homer1 and actin (phalloidin, in confocal mode) showed ERGIC53 at the dendritic shaft and at postsynapses in close proximity to Homer1. Authors discussed the interpretation of ERGIC53 (one of the POI, proteins of interest); "Nevertheless, in our experimental setup all POIs could be identified unambiguously in the dendritic shafts and at post-synaptic compartments. According to previous studies, the ERGIC was proposed to be rarely present in dendritic spines (PMID:28875935). However, it should be noted that in electron microscopy the ERGIC resembles endosomes, which are often found in spines, and therefore this similarity might lead to misinterpretation of the images (PMID:28559323). Furthermore, fine ERGIC53 puncta could be easily overseen or mislocalised when using diffraction-limited light microscopy. " |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5219 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |