| Annotated protein: | Sharpin (Shank-associated RH domain-interacting protein). Gene symbol: SHARPIN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9EQL9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SHARPIN |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | cerebellum, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Lim S, et al. "Sharpin, a novel postsynaptic density protein that directly interacts with the shank family of proteins" Mol Cell Neurosci. 2001 Feb;17(2):385-97 PMID:11178875 |
| Figure(s): | Figure 4, 5, 6, 8 |
| Annotation description: | Figure 4c: SHARPIN is enriched in PSD biochemical fractions from rat brain (similar to Shank). Figure 5: reverse-IP in COS7 cells and rat cerebella showed that SHARPIN and SHANK1 can interact. Figure 6, 8: immunofluorescence imaging showed SHARPIN and SHANK1 partially co-localized at excitatory postsynapses in cultured hippocampal neurons and cerebellum tissue. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5214 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |