| Annotated protein: | Dedicator of cytokinesis 4. Gene symbol: DOCK4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I5ZNR8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DOCK4 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ueda S, et al. "Rac GEF Dock4 interacts with cortactin to regulate dendritic spine formation" Mol Biol Cell. 2013 May;24(10):1602-13 PMID:23536706 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1d-j: confocal imaging of immunofluorescence staining for DOCK4, PSD95 (DLG4) and synaptophysin (SYP) in hippocampal neurons showed localization of DOCK4 to the postsynapse. Figure 2d-g, 3: shRNA knockdown of DOCK4 in hippocampal neurons impaired dendritic spine formation. Specific shRNA targeting DOCK4 domains showed that the GEF activity and the proline-rich region are required for Dock4-mediated spine formation. authors conclude: "These results suggest that Dock4 positively regulates dendritic spine formation through activation of Rac in hippocampal neurons." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5213 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |