| Annotated protein: | Dedicator of cytokinesis protein 10 (Zizimin-3). Gene symbol: DOCK10. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BZN6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DOCK10 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Jaudon F, et al. "The RhoGEF DOCK10 is essential for dendritic spine morphogenesis" Mol Biol Cell. 2015 Jun 1;26(11):2112-27 PMID:25851601 |
| Figure(s): | Figure 5 |
| Annotation description: | Figure 4: overexpressed Flag-DOCK10 (mouse) is localized in dendritic spines of hippocampal neurons. Figure 5: "ShRNA-mediated depletion of DOCK10 led to a dramatic decrease in the number of spines along the dendritic shaft (Figure 5, A and B). This defect was accompanied by a decrease in spine head size in the remaining spines (Figure 5C). In addition, the postsynaptic density protein Homer-1 was hardly detected by immunofluorescence in the remaining protrusions of DOCK10-depleted neurons as compared with control neurons, confirming that spines devoid of DOCK10 are not properly formed (Figure 5, D and E). To confirm the requirement of DOCK10 for spine formation, we performed rescue experiments by reintroducing a shRNA-resistant DOCK10 construct into DOCK10-depleted hippocampal neurons. This construct was designed to be resistant only to shA DOCK10 and not to shB, as demonstrated in Supplemental Figure S2A (see Materials and Methods). As shown in Figure 5, F and G, wild-type (wt) DOCK10shR was able to rescue the decrease in both spine number and spine head size induced by shA-mediated DOCK10 depletion, demonstrating its requirement for spine morphogenesis." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5211 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |