| Annotated protein: | Rho-related GTP-binding protein RhoG. Gene symbol: RHOG. Taxonomy: Homo sapiens (Human). Uniprot ID: P84095 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RHOG |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kim JY, et al. "The RhoG/ELMO1/Dock180 signaling module is required for spine morphogenesis in hippocampal neurons" J Biol Chem. 2011 Oct 28;286(43):37615-24 PMID:21900250 |
| Figure(s): | Figure 5, 6, 7 |
| Annotation description: | Figure 6d: overexpression of constitutively active RhoG-Q61L in hippocampal neurons showed localization to dendrites and spines apposed to presynaptic terminals labeled by anti-synapsin1 (SYN1). Figure 6a-c: overexpression of EGFP wild type RhoG, a dominant negative EGFP-RhoG T17N, and a constitutively active EGFP-RhoG Q61L showed that activation of RhoG causes increased spine density and enlargement of the spine head. Figure 5d,e: shRNA knockdown of RhoG abolished dendritic spine formation in cultured hippocampal neurons. Figure 7: "To determine whether RhoG functions through ELMO1/Dock180/Rac in spine morphogenesis, we depleted ELMO1 in the context of the constitutively active RhoG-Q61L. As seen in Fig. 7, a and b, we were able to reverse the RhoG-Q61L phenotype by depleting ELMO1. In addition, overexpression of ELMO1 was able to rescue the defects caused by RhoG depletion (Fig. 7, c and d). Finally, RacN17 was able to reverse the phenotype induced by RhoG Q61L (Fig. 7, e and f). Taken together, these results argue that RhoG functions upstream of ELMO1/Dock180/Rac in spine morphogenesis." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5210 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |