| Annotated protein: | Dedicator of cytokinesis protein 1 (180 kDa protein downstream of CRK) (DOCK180). Gene symbol: DOCK1. Taxonomy: Homo sapiens (Human). Uniprot ID: Q14185 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DOCK1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kim JY, et al. "The RhoG/ELMO1/Dock180 signaling module is required for spine morphogenesis in hippocampal neurons" J Biol Chem. 2011 Oct 28;286(43):37615-24 PMID:21900250 |
| Figure(s): | Figure 1, 3, 4 |
| Annotation description: | Figure 1b, 3e: immunofluorescence imaging of hippocampal neurons stained for endogenous Dock180 (DOCK1) and PSD95 (DLG4) showed partial co-localization. Stainings of Dock180 were apposed to presynaptic synapsin-1 (SYN1). Overexpressed FLAG-Dock180 localized to dendrites and spines. Figure 4: ELMO and Dock180 (DOCK1) form a complex in hippocampal neurons and function through activating Rac1. Overexpressed Dock180 increased spinogenesis. Figure 5-7: RhoG is required for spine formation, its activation leads to increased spine head size and functions through the ELMO1/Dock180/Rac pathway. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5208 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |