| Annotated protein: | E3 ubiquitin-protein ligase Mdm2 (EC 2.3.2.27). Gene symbol: MDM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I6G5V3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MDM2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Annotated paper: | Bianchetta MJ, et al. "Cyclin-dependent kinase 5 regulates PSD-95 ubiquitination in neurons" J Neurosci. 2011 Aug 17;31(33):12029-35 PMID:21849563 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 1: Reduced Cdk5 activity via p35 knockout promoted PSD95 (DLG4) ubiquitination. Figure 2: Reduced Cdk5 activity via p35 knockout increased interaction and colocalization of Mdm2 with PSD95. In p35 knockout mice, the amount of Mdm2 obtained through PSD95 pulldown is increased as compared to wildtype. Confocal imaging of immunofluorescence labeling for Mdm2 and PSD95 showed increased co-localization in cultured hippocampal neurons upon treatment with roscovitine (Cdk5 inhibitor). |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | reverse-IP showed PSD95 (DLG4) interacts with Mdm2 in synaptic plasma membrane fractions. Co-localization for Mdm2 and synapsin-1 (SYN1) was observed in cultured hippocampal neurons. @ PMID:14642282 |
| SynGO annotation ID: | 5198 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |