| Annotated protein: | Sorting nexin-27. Gene symbol: SNX27. Taxonomy: Homo sapiens (Human). Uniprot ID: Q96L92 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SNX27 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse maturation (GO:0090128) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Huo Y, et al. "Overexpression of Human SNX27 Enhances Learning and Memory Through Modulating Synaptic Plasticity in Mice" Front Cell Dev Biol. 2020 Nov 27;8:595357 PMID:33330482 |
| Figure(s): | Figure 6 |
| Annotation description: | Figure 4: Overexpression of human SNX27 promoted synaptic plasticity in hSNX27 mice. "In input-output response, a significant increase in the amplitude of the evoked excitatory postsynaptic current (eEPSC) was detected in hSNX27 Tg mice relative to WT mice (Figure 4A). In addition, paired-pulse facilitation remains unchanged in hSNX27 Tg mice compared to WT mice (Figure 4B). Consistent with input-output response, enhanced LTP at hippocampal CA1 region was observed in hSNX27 mice compared to WT mice (Figure 4C), suggesting that SNX27 overexpression increases activity-dependent synaptic plasticity in hippocampus. Furthermore, hSNX27 Tg mice showed enhanced mEPSC amplitude but unaffected mEPSC frequency compared to WT mice (Figure 5A), suggesting that SNX27 overexpression enhances the amount and/or the function of postsynaptic AMPA and NMDA receptors. However, both amplitude and frequency of mIPSC were similar between hSNX27 Tg mice and WT littermates (Figure 5B). Moreover, excitation/inhibition balance (E/I ratio) was not affected by SNX27 overexpression in hSNX27 transgenic mice (Supplementary Figure 4). Together, these results demonstrate that SNX27 overexpression enhances excitatory synaptic transmission and synaptic plasticity in hSNX27 Tg mice." Figure 6: Overexpression of Human SNX27 Affected Synapse Formation. "Enhanced synaptic function is usually accompanied by dynamic alterations in synaptic density or structure, such as the formation of new synapses or the consolidation of existing synapses (Batool et al., 2019). We first performed Nissl staining and found that SNX27 overexpression did not affect the gross morphology of cortex and hippocampus of hSNX27 Tg mice (Figure 6A). To determine whether SNX27 overexpression affects synapse formation, we consequently performed Golgi staining and found an increased density of mature synapses and a decreased number of immature synapses in hippocampal neurons of 2-month-old hSNX27 Tg mice compared with that in WT littermates (Figure 6B), suggesting that SNX27 plays a key role in regulating synaptic structure and function without affecting gross brain morphology." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5192 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |