| Annotated protein: | Potassium voltage-gated channel subfamily H member 1 (Ether-a-go-go potassium channel 1) (EAG channel 1) (EAG1) (r-eag) (Voltage-gated potassium channel subunit Kv10.1). Gene symbol: KCNH1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q63472 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KCNH1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Annotated paper: | Chuang CC, et al. "The punctate localization of rat Eag1 K+ channels is conferred by the proximal post-CNBHD region" BMC Neurosci. 2014 Feb 4;15:23 PMID:24495567 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 2a-c: confocal imaging of immunofluorescence labeling for Eag1 (KCNH1) and PSD95 (DLG4) in cultured hippocampal neurons from rat brain showed partial co-localization (~65%). Figure 2d: biochemical fractions of rat brains showed Eag1 in synaptic fractions P2, synaptosomes, PSD1 and PSD2 (relative levels are; synaptosome = PSD1 > PSD2, whereas PSD95 is enriched SPM < PSD1 < PSD2. But note that Eag1 is known to also localize to the presynapse). Figure 3,5: overexpression of GFP-Eag1 constructs and mutant variants showed the A723-R807 segment of the protein sequence was required for postsynaptic localization. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | generation of the eag1/KCNH1 antibody and co-localization with synaptic markers in hippocampal neurons @ PMID:15706225 |
| SynGO annotation ID: | 5191 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |