| Annotated protein: | Neurabin-2 (Neurabin-II) (Protein phosphatase 1 regulatory subunit 9B) (Spinophilin). Gene symbol: PPP1R9B. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6R891 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPP1R9B |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | striatum |
| Annotated paper: | Feng J, et al. "Spinophilin regulates the formation and function of dendritic spines" Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):9287-92 PMID:10922077 |
| Figure(s): | Figure 3, table 1 |
| Annotation description: | Figure 3: Spinophilin (aka. neurabin-2, gene PPP1R9B) knockout resulted in a decreased spine density in caudatoputamen of young P15 mice (and a small but not significant increase) in adult mice. The size, shape, and variability of spines was knock affected in knockouts. "To further characterize dendritic morphology in the knockout mice, we cultured hippocampal neurons at low density to estimate the number of spine-like protrusions (or filopodia) and synapses. Cells were stained at various intervals with the F-actin stain, phalloidin, or anti-tubulin to determine the number of spine-like protrusions or with anti-synaptophysin to determine the number of synaptic terminals. In contrast to high-density cultures (37), in our low-density cultures, filopodia appeared somewhere between 14 and 21 days in vitro. In cultures from the knockout mice, these filopodia were present much earlier in development and in much larger numbers (Table 1). However, the number of synaptophysin puncta was similar in the knockout and wild-type cultures at each time point (Table 1)." Figure 4: Regulation of AMPA and NMDA receptors Is altered in Spinophilin knockout mice. Figure 5: Spinophilin knockout mice exhibit reduced LTD, but normal LTP. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Neurabin-II/spinophilin is an actin filament-binding protein with one pdz domain. Neurabin-II/spinophilin was enriched at the postsynaptic density fraction in rat brain. Immunofluorescence microscopic analysis revealed that neurabin-II was highly concentrated at the synapse in primary cultured rat hippocampal neurons and at the cadherin-based cell-cell adhesion sites in Madin-Darby canine kidney cells. @ PMID:9452470 |
| SynGO annotation ID: | 5187 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |