| Annotated protein: | Neuronal migration protein doublecortin (Doublin) (Lissencephalin-X) (Lis-X). Gene symbol: DCX. Taxonomy: Mus musculus (Mouse). Uniprot ID: O88809 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DCX |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Martineau FS, et al. "Correct Laminar Positioning in the Neocortex Influences Proper Dendritic and Synaptic Development" Cereb Cortex. 2018 Aug 1;28(8):2976-2990 PMID:29788228 |
| Figure(s): | Figure 7, 8 |
| Annotation description: | Figure 7: "we checked for a potential effect of Dcx-pKD (postnatal knockdown) on spine density and morphology. In Dcx-pKD neurons properly placed in layer V, spine density and length was similar to the one in mismatch neurons. However, spines had a slightly wider head and a slightly wider neck in Dcx-pKD neurons, suggesting a role for Dcx in spine formation. In order to play such a role, Dcx should be present in the synaptic compartment during the first postnatal weeks. In cortical layer V neurons, very little dendritic spines are visible during this period making difficult any immunohistochemical evaluation. Therefore, we decided to analyze by Western blot cytosolic and synaptosomal fractions isolated from P10, P15, and P20 rat neocortex and found that Dcx was enriched in the synaptosomal as compared with the cytosolic fraction." Figure 8: Miniature excitatory currents are impaired after postnatal knockdown of Dcx. "Because Dcx seems involved in spine formation, glutamatergic synaptogenesis could also be affected by Dcx postnatal knockdown. ... Overall, our data show that the amount of PSD scaffold is mostly unaffected in Dcx-pKD neurons." "Nevertheless, the spine alterations reported above could be associated with altered synaptic transmission. We checked this point by recording mEPSCs from Dcx-pKD neurons and mismatch neurons. In Dcx-pKD neurons, the cumulative frequency curve of mEPSC inter-event interval was shifted towards higher values, revealing a decreased frequency of mEPSCs in Dcx-pKD neurons compared with mismatch neurons. In addition, although the cumulative frequency curve of mEPSC amplitude revealed no change in Dcx-pKD neurons compared with mismatch neurons, the cumulative frequency curve of mEPSC half-width was slightly but significantly shifted towards lower values in Dcx-pKD neurons. Altogether, these data are consistent with the notion that Dcx, in addition to its known role on migration, influences spine formation, as well as the proper development of functional glutamatergic synapses." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5186 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |