| Annotated protein: | Serine/threonine-protein kinase DCLK1 (EC 2.7.11.1) (Doublecortin-like and CAM kinase-like 1) (Doublecortin-like kinase 1). Gene symbol: DCLK1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JLM8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DCLK1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Shin E, et al. "Doublecortin-like kinase enhances dendritic remodelling and negatively regulates synapse maturation" Nat Commun. 2013;4:1440 PMID:23385585 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1i-j: immunofluorescence staining for endogenous PSD95 (DLG4) and overexpressed GFP-DCLK1 in cultured hippocampal neurons showed DCLK1 localization to dendrites and dendritic spines. Figure 4: excitatory postsynaptic development is negatively regulated by (long form) DCLK1. Overexpression of GFP-DCLK1 reduced spine density and mushroom-spine numbers. shRNA knockdown of DCLK1 increased spine density, volume and mushroom-spine numbers. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5184 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |