| Annotated protein: | Serine/threonine-protein kinase DCLK1 (EC 2.7.11.1) (Doublecortin-like and CAM kinase-like 1) (Doublecortin-like kinase 1). Gene symbol: DCLK1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JLM8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DCLK1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Shin E, et al. "Doublecortin-like kinase enhances dendritic remodelling and negatively regulates synapse maturation" Nat Commun. 2013;4:1440 PMID:23385585 |
| Figure(s): | Figure 1 |
| Annotation description: | Figure 1a, S1: generation of a polyclonal pan-DCLK-antibody against the DCLK1 DCX domain. Targets a region with high homology between DCLK1 and DCLK2, has higher affinity for GFP-DCLK1 than GFP-DCLK2, and was validated with DCLK1-/- and DCLK2-/- knockout mouse controls. Figure 1i-j: immunofluorescence staining for endogenous PSD95 (DLG4) and overexpressed GFP-DCLK1 in cultured hippocampal neurons showed DCLK1 localization to dendrites and dendritic spines. Figure 1k: DCLK was enriched in SV, synaptic plasma membrane and PSD biochemical fractions. Figure 1l: pulldown of anti-DCLK in adult brain yields PSD95 (DLG4). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5183 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |