| Annotated protein: | Synapse differentiation-inducing gene protein 1 (SynDIG1) (Dispanin subfamily C member 2) (DSPC2) (Transmembrane protein 90B). Gene symbol: SYNDIG1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q58DZ9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYNDIG1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kalashnikova E, et al. "SynDIG1: an activity-regulated, AMPA- receptor-interacting transmembrane protein that regulates excitatory synapse development" Neuron. 2010 Jan 14;65(1):80-93 PMID:20152115 |
| Figure(s): | Figures 4-8 |
| Annotation description: | Figure 3: SynDIG1 interacts with GluA2 in heterologous cells. Figure 4, 5: shRNA knockdown of SynDIG1 reduced the number of excitatory synapses in dissociated rat hippocampal neurons and decreased the frequency and amplitude of miniature excitatory postsynaptic current (mEPSC). Figure 6, 7: Conversely, SynDIG1 overexpression promoted excitatory synapse development and increased mEPSC frequency and amplitude. Figure 8: SynDIG1 distribution at excitatory synapses is activity regulated. "Sodium channel-dependent action potentials in hippocampal neurons were blocked by addition of tetrodotoxin (TTX) at 10 DIV. Upon activity blockade for two to four days, SynDIG1 immunoreactivity redistributed from diffuse and punctate staining in dendrite shafts to bright clusters, presumably spines, protruding from dendrites (Figure 8A). The overall level of SynDIG1 protein did not change in neurons treated with TTX compared with vehicle as assessed by immunobloting with anti-SynDIG1 mAb (Figure S8A). Under control conditions, SynDIG1 is enriched 2.5-fold in spines relative to shafts; that enrichment increases significantly to 7.0-fold after TTX treatment (Figure 8B). In contrast, SynDIG1 puncta density in the presence or absence of TTX does not change (Figure 8C). Thus, SynDIG1 distribution but not synthesis is regulated by synaptic activity in hippocampal neurons." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antibody (detection) Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5180 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |