| Annotated protein: | Synapse differentiation-inducing gene protein 1 (SynDIG1) (Dispanin subfamily C member 2) (DSPC2) (Transmembrane protein 90B). Gene symbol: SYNDIG1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q58DZ9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYNDIG1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kalashnikova E, et al. "SynDIG1: an activity-regulated, AMPA- receptor-interacting transmembrane protein that regulates excitatory synapse development" Neuron. 2010 Jan 14;65(1):80-93 PMID:20152115 |
| Figure(s): | Figure 2, S2, 8 |
| Annotation description: | Figure 2c: Cultured hippocampal neurons were immunostained for endogenous SynDIG1, SAP102 (DLG3), vGlut1, GluA2 (GRIA2), vGAT and gephyrin. SynDIG1 partially co-localized with excitatory, but not inhibitory, synaptic marker proteins. Postsynaptic localization for SynDIG1 was apparent in surface-SynDIG1 co-stainings with PSD95 and vGlut1 (Fig. S4b) in dissociated rat hippocampal neurons transfected at 4DIV. Figure S4c: SynDIG1 and PSD95 are co-enriched in various PSD biochemical fractions prepared from mouse P14 brain. Figure 8: Immunostaining for SynDIG1 and MAP2 in dissociated rat hippocampal neurons at 10 DIV showed clear dendritic puncta. Figure 3: SynDIG1 interacts with GluA2 in heterologous cells. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5179 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |