| Annotated protein: | Protein Smaug homolog 1 (Smaug 1) (Sterile alpha motif domain-containing protein 4A). Gene symbol: SAMD4A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B5DF21 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SAMD4A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Baez MV, et al. "Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation" J Cell Biol. 2011 Dec 26;195(7):1141-57 PMID:22201125 |
| Figure(s): | Figure 9 |
| Annotation description: | Figure 3: Smaug1 (SAMD4A) localized to postsynapses in hippocampal neurons . Figure 9: confocal imaging of hippocampal neurons subjected to siRNA knockdown of Smaug1 (SAMD4A) showed reduced synapse size and number. "We found that both PSD95 and synapsin clusters were smaller and more numerous than in control or siNT-treated cells (Fig. 9 C and not depicted). ... PSD95-containing synapses in hippocampal cells are mostly excitatory, and are located in dendritic spines. To visualize dendritic spines, neurons were transfected with ECFP and stained for PSD95 (Fig. 9 F). As expected, PSD95 clusters were located at the tip of dendritic spines. Remarkably, dendritic spines were longer and thinner in siSmaug1-treated cells, thus resembling an immature morphology." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5176 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |