| Annotated protein: | Protein Smaug homolog 1 (Smaug 1) (Sterile alpha motif domain-containing protein 4A). Gene symbol: SAMD4A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: B5DF21 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SAMD4A |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Baez MV, et al. "Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation" J Cell Biol. 2011 Dec 26;195(7):1141-57 PMID:22201125 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3a,b: Cultured hippocampal neurons were immunostained for endogenous Smaug1 (SAMD4A), PSD95 (DLG4) and synapsin. Smaug1 partially co-localized with PSD95 in closer proximity than presynaptic marker protein synapsin. Figure 3e: Smaug1 and PSD95 were enriched in PSD biochemical fractions from adult hippocampus as compared to input lysate and Synaptoneurosomes. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Smaug1 antibody @ PMID:16221671 |
| SynGO annotation ID: | 5175 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |