| Annotated protein: | Cannabinoid receptor 2 (CB-2) (CB2) (mCB2). Gene symbol: CNR2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P47936 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CNR2 |
| Ontology domain: | Biological Process |
| SynGO term: | trans-synaptic signaling by endocannabinoid, modulating synaptic transmission (GO:0099553) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Stempel AV, et al. "Cannabinoid Type 2 Receptors Mediate a Cell Type-Specific Plasticity in the Hippocampus" Neuron. 2016 May 18;90(4):795-809 PMID:27133464 |
| Figure(s): | Figure 5, 6 |
| Annotation description: | Figure 5: The mixed CBR agonists 2-AG and WIN,55212-2 (WIN) as well as the CB2R-specific agonist HU-308 (HU) all strongly hyperpolarized CA3 PCs in WT mice. Specificity of targeting CB2R was validated with CB2R-/- mice. Neither of the drugs led to a hyperpolarization in these animals, strongly arguing for a purely CB2R-dependent mechanism (Figures 5D-5F). Figure 6: "As CBRs are G protein-coupled receptors (GPCRs), we confirmed that the hyperpolarization is mediated by a G protein-dependent cascade by performing experiments with 0.5 mM GDPβS, a non-hydrolyzable GDP analog that blocks G protein-coupled activity. The inclusion of GDPβS into the pipette abolished the pharmacologically induced hyperpolarization within the manipulated neuron (Figures 6A and 6B), supporting the idea of a cell-intrinsic, G protein-dependent mechanism." "Highlights - CB2Rs are expressed in hippocampal principal neurons - CB2Rs mediate a cell type-specific self-inhibitory plasticity in CA3/CA2 PCs - CB2Rs reduce the spike probability of CA3 PCs and alter gamma oscillations in vivo - CB2Rs act complementary to presynaptic CB1Rs" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5174 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |