| Annotated protein: | Diacylglycerol lipase-alpha (DAGL-alpha) (DGL-alpha) (EC 3.1.1.116) (Neural stem cell-derived dendrite regulator) (Sn1-specific diacylglycerol lipase alpha). Gene symbol: DAGLA. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6WQJ1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DAGLA |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hypothalamus |
| Annotated paper: | Reguero L, et al. "Subcellular localization of NAPE-PLD and DAGL-alpha in the ventromedial nucleus of the hypothalamus by a preembedding immunogold method" Histochem Cell Biol. 2014 May;141(5):543-50 PMID:24346263 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 1: DAGL-α (DAGLA) antibody specificity validation using knockout mice. Figure 3: immunogold EM imaging of the ventromedial nucleus of the hypothalamus showed DAGL-α (DAGLA) particles at the membranes of dendrites and dendritic spines. 54.4 % of dendrites and 44.4 % of dendritic spines were DAGL-α immunopositive. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5168 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |