| Annotated protein: | N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (N-acyl phosphatidylethanolamine phospholipase D) (NAPE-PLD) (NAPE-hydrolyzing phospholipase D) (EC 3.1.4.54). Gene symbol: NAPEPLD. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BH82 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NAPEPLD |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic membrane (GO:0042734) |
| Synapse type(s): | hypothalamus |
| Annotated paper: | Reguero L, et al. "Subcellular localization of NAPE-PLD and DAGL-alpha in the ventromedial nucleus of the hypothalamus by a preembedding immunogold method" Histochem Cell Biol. 2014 May;141(5):543-50 PMID:24346263 |
| Figure(s): | Figure 2 |
| Annotation description: | Figure 1: NAPEPLD antibody specificity validation using knockout mice. Figure 2: immunogold EM imaging of the ventromedial nucleus of the hypothalamus showed NAPEPLD particles at the plasma membrane of both pre- and post-synapses. Labeling was also observed inside the neuronal profiles (Fig. 2f), frequently associated with intracellular organelles such as the smooth endoplasmic reticulum (Fig. 2c). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5166 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |